无动物源成分重组细胞因子

重组人骨形态发生蛋白-13 /Recombinant BMP-13，Human，Animal-Free

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产品编号	优惠促销	规格	价格	货期
CM075-5HP	买一赠一	5 ug	￥600.00	现货
CM075-20HP		20 ug	￥1500.00	现货
CM075-100HP		100 ug	￥4500.00	现货
CM075-500HP		500 ug	￥13000.00	请询
CM075-1000HP		1 mg	￥20000.00	请询

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产品简介

背景描述	Bone Morphogenetic Protein-13 (BMP-13), known as Growth differentiation factor 6 (GDF6), is an extracellular multifunctional cytokine that is also a member of the TGFβ family. BMP-13 can bind with the TGFβ receptor and induce SMAD protein signal transduction. BMP-13 is a regulatory protein that can affect the growth and differentiation of developing embryos. Moreover, it plays a role in regulating the patterning of the ectoderm and controlling eye development.
别称	bone morphogenetic protein 13, subunit (CLMF p35), NK cell Stimulating Factor Chain 1, BMP13
蛋白编码	Q6KF10
分子量	The protein has a calculated MW of 14.50 kDa. The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>98% as determined by SDS-PAGE.
生物活性	Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is 63-240 ng/mL.
内毒素检测	<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列	MTAFASRHGKRHGKKSRLRCSKKPLHVNFKELGWDDWIIAPLEYEAYHCEGVCDFPLRSHLEPTNHAIIQTLMNSMDPGSTPPSCCVPTKLTPISILYIDAGNNVVYKQYEDMVVESCGCR with polyhistidine tag at the C-terminus.
蛋白标签	His Tag (C-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示


SDS- PAGE analysis of recombinant human BMP-13	Human BMP-13, His Tag, E. coli induced alkaline phosphatase production by ATDC5 cells, with the ED₅₀ at 63 ng/mL.

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