| 背景描述 | IGFBP7, also known as mac25, prostacyclin-stimulating factor (PSF), tumor adhesion factor (TAF), and angiomodulin (AGM), is a secreted protein that belongs to the insulin-like growth factor (IGF)-binding protein (IGFBP) family. In contrast to the other family members, IGFBP7 binds IGFs through the N-terminal domain with low affinity. IGFBP7 is expressed in various human tissues, including the brain, liver, pancreas, and skeletal muscle, and is secreted into circulation. IGF-BP7 modulates many biological functions, such as protein synthesis, proliferation, anti-apoptosis, and cell survival. Moreover, it has been reported that IGFBP7 promotes cardiac senescence through IGF-1R/IRS/AKT-dependent suppression of FOXO3a, inhibiting DNA repair, subsequently the leading to the progression of Heart failure. Human and mouse IGFBP7 are highly homologous, sharing 94% a.a. sequence identity. |
| 别称 | IGF-binding protein 7, IBP-7, IGFBP-7, IGFBP-rP1, MAC25, PSF(Prostacyclin-stimulating factor), TAF(Tumor-derived adhesion factor) |
| 蛋白编码 | Q16270 |
| 表达系统 | HEK293 |
| 纯度 | >95% as determined by SDS-PAGE. |
| 生物活性 | Testing in process |
| 内毒素检测 | <1 EU per 1 μg of the protein by the LAL method. |
| 蛋白序列 | A DNA sequence encoding Human IGFBP7 (#Q16270) (Ser27 – Leu282) was expressed with polyhistidine-SUMO tag at the N-terminus. |
| 蛋白标签 | His-SUMO Tag (N-term) |
| 产品形式 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us. |
| 产品应用 | Cell Culture |
| 分子量 | The protein has a calculated MW of 38.18 kDa. The protein migrates as 55 kDa under reducing condition (SDS-PAGE analysis). |
1. Before opening, centrifuge at 3000 rpm for 5 mins.
2. Initial Reconstitution
· Reconstitute the Lyophilized Protein in sterile H₂O to a concentration of 100-200 μg/mL.
· Then, incubate it at room temperature for at least 20 mins to ensure sufficient dissolution.
Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
· Store at 2°C to 8°C for up to 1 week.
3. Extended Storage
· After the initial reconstitution, further dilute the reconstituted protein in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/ml.
· Prepare aliquots (≥20 μl).
· Store at -20°C or -80°C for 3 to 6 months.
Avoid repeated freeze-thaw cycles.
| 储存 | Lyophilized Protein: Store at -20°C for 1 year. Initial Reconstitution: Store at 2°C to 8°C for up to 1 week. Extended Storage: Store at -20°C or -80°C for 3 to 6 months with a carrier protein or stabilizer. |
| 运输 | Blue Ice |
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| SDS-PAGE analysis of recombinant human IGFBP7 |
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