| 背景描述 | R-Spondin 3 (RSPO3) is a secreted growth factor that enhances canonical Wnt/β-catenin signaling through interaction with LGR4/5/6 receptors and inhibition of RNF43/ZNRF3-mediated receptor turnover. RSPO3 plays essential roles in stem cell maintenance, angiogenesis, vascular development, tissue regeneration, and embryonic morphogenesis. It is widely used in organoid culture systems to support stem cell proliferation and epithelial growth. Aberrant RSPO3-mediated Wnt signaling has also been implicated in cancer progression and tumor microenvironment regulation. |
| 别称 | R-Spondin 3, RSPO3, Roof plate-specific Spondin-3, LGR-binding growth factor, PWTSR, THSD2, Protein with thromboSpondin type 1 repeat, ThromboSpondin type-1 domain-containing protein 2 |
| 蛋白编码 | Q9BXY4 |
| 表达系统 | CHO |
| 纯度 | >90% as determined by SDS-PAGE. |
| 生物活性 | R-Spondin 3 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED₅₀ for this effect is < 3 μg/mL. |
| 内毒素检测 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 蛋白序列 | A DNA sequence encoding Human R-Spondin 3 Protein(#Q9BXY4) (Gln22-His272) was expressed with polyhistidine-SUMO tag at the N-terminus. |
| 蛋白标签 | His-SUMO Tag (N-term) |
| 产品形式 | Liquid. Phosphate buffered saline, pH 7.4. If you have any concerns or special requirements, please confirm with us. |
| 产品应用 | Cell Culture |
| 浓度 | Please refer to the product label. |
| 分子量 | The protein has a calculated MW of 48.4 kDa. The protein migrates as 62-70 kDa under reducing condition (SDS-PAGE analysis). |
1. Before opening, centrifuge at 3000 rpm for 5 mins.
2. Stock Solution
· Store at 2°C to 8°C for up to 1 week, -20°C or -80°C for 12 months.
3. Dilution
· Further dilute the stock solution in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/mL.
· Prepare aliquots (≥20 μL).
· Store at -20°C or -80°C for 3 to 6 months.
Avoid repeated freeze-thaw cycles.
| 储存 | Stock Solution: Store at 2°C to 8°C for up to 1 week, -20°C or -80°C for 12 months. Dilution: Store at -20°C or -80°C for 3 to 6 months with a carrier protein or stabilizer. |
| 运输 | Blue Ice |
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| SDS-PAGE analysis of recombinant human R-Spondin 3 | Activity analysis of recombinant Human R-Spondin 3, His-SUMO Tag, CHO. R-Spondin 3 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED₅₀ for this effect is < 3 μg/mL. |
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