| 背景描述 | Insulin Like Growth Factors 1 (IGF-I) is a 7.79 kDa member of the Insulin-like Growth Factors with 71 amino acid residues. IGF-I is mainly expressed from liver, adipose tissue, cervi, endometrial stromal cells, leydig cells, and can be isolated from plasma. IGF-I is mediating the protein anabolic and promoting effect of pituitary growth hormone. IGF-I also affects metabolism of glycogen, DNA synthesis and glucose uptake via binding to IGF-I receptor. |
| 别称 | insulin-like growth factor-I, IGF-1, Npt2B |
| 蛋白编码 | P16545 |
| 分子量 | The protein has a calculated MW of 8.59 kDa. The protein migrates below 11 kDa under reducing condition (SDS-PAGE analysis). |
| 表达系统 | Escherichia coli |
| 纯度 | >98% as determined by SDS-PAGE. |
| 生物活性 | Testing in process |
| 内毒素检测 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 蛋白序列 | MGPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA with polyhistidine tag at the C-terminus. |
| 蛋白标签 | His Tag (C-term) |
| 产品形式 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us. |
| 产品应用 | Cell Culture |
| 储存 | Lyophilized protein: Store at -20°C for 1 year. After reconstitution: · Store at 2°C to 8°C for up to 1 week. · For extended storage (beyond 1 week), dilute the reconstituted protein in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/ml. Prepare aliquots (≥20 μl) and store at -20°C or -80°C for 3 to 6 months. Avoid repeated freeze-thaw cycles. |
| 运输 | Blue Ice |
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| SDS- PAGE analysis of recombinant swine IGF-I |
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