| 背景描述 | Wnt3a is a secreted glycoprotein that activates the canonical Wnt/β-catenin signaling pathway, playing a crucial role in embryonic development, tissue homeostasis, and injury repair. However, its experimental application is hindered by poor solubility and cross-reactivity with multiple Frizzled receptors. To address these challenges, engineered Wnt3a surrogate proteins have been developed. These surrogates are water-soluble and designed to specifically bind to targeted Frizzled receptors, effectively mimicking natural Wnt3a activity with enhanced precision. This specificity and improved solubility make Wnt3a surrogates valuable tools in research areas such as organoid culture, stem cell maintenance, and studies of developmental biology. |
| 别称 | Wnt3a Surrogate, Wingless-type MMTV integration site family member 3A Surrogate, WNT3A |
| 分子量 | The protein has a calculated MW of 58.5 kDa. The protein migrates as 55-75 kDa under reducing condition (SDS-PAGE analysis). |
| 表达系统 | HEK293 cell |
| 纯度 | >95% as determined by SDS-PAGE. |
| 生物活性 | Measured by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <20 ng/mL. |
| 内毒素检测 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 蛋白序列 | Human Wnt-3a Surrogate protein is expressed from HEK293 with human IgG1 Fc tag at the C-terminus. |
| 蛋白标签 | Human IgG1 Fc Tag (C-term) |
| 支原体 | Not detected |
| 产品形式 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us. |
| 产品应用 | Cell Culture |
1. Before opening, centrifuge at 3000 rpm for 5 mins.
2.Initial Reconstitution
· Reconstitute the Lyophilized Protein in sterile H₂O to a concentration of 100-200 μg/mL.
· Then, incubate it at room temperature for at least 20 mins to ensure sufficient dissolution.
Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
· Store at 2°C to 8°C for up to 1 week.
3.Extended Storage
· After the initial reconstitution, further dilute the reconstituted protein in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/ml.
· Prepare aliquots (≥20 μl).
· Store at -20°C or -80°C for 3 to 6 months.
Avoid repeated freeze-thaw cycles.
| 储存 | Lyophilized Protein: Store at -20°C for 1 year. Initial Reconstitution: Store at 2°C to 8°C for up to 1 week. Extended Storage: Store at -20°C or -80°C for 3 to 6 months with a carrier protein or stabilizer. |
| 运输 | Blue Ice |
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| SDS- PAGE analysis of recombinant human Wnt-3a Surrogate | Human Wnt-3a Surrogate, Fc Tag, HEK293 induced alkaline phosphatase production by ATDC5 cells, with the ED₅₀ <20 ng/mL. |
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