| 背景描述 | Insulin is a peptide hormone consisting of a double-chain structure (α, β) secreted by pancreatic β cells. It is the only known endogenous protein hormone capable of lowering blood sugar levels. Insulin not only regulates the intake, utilization, and storage of glucose, amino acids, and fatty acids in cells but also promotes the synthesis of glycogen, fats, and proteins. |
| 别称 | INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin |
| 蛋白编码 | P01308 |
| 分子量 | The protein has a calculated MW of 5.81 kDa. The protein migrates as 5 kDa under reducing condition (SDS-PAGE analysis). |
| 表达系统 | Escherichia coli |
| 纯度 | >95% as determined by SDS-PAGE. |
| 生物活性 | Testing in process |
| 内毒素检测 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 蛋白序列 | A DNA sequence encoding Human Insulin Protein(#P01308) (Phe25-Asn110) was expressed. |
| 蛋白标签 | Tag Free |
| 产品形式 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us. |
| 产品应用 | Cell Culture |
1. Before opening, centrifuge at 3000 rpm for 5 mins.
2.Reconstitution
· Reconstitute the Lyophilized Protein in sterile 10 mM HCl to a concentration of 1-2 mg/mL.
· Then, incubate it at room temperature for at least 20 mins to ensure sufficient dissolution.
Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
· Prepare aliquots.
· Store at -20°C or -80°C for 1 month.
Avoid repeated freeze-thaw cycles.
| 储存 | Lyophilized Protein: Store at -20°C for 1 year. Reconstitution: Store at -20°C or -80°C for 1 month. |
| 运输 | Blue Ice |
 |
| SDS-PAGE analysis of recombinant human Insulin |
暂无参考文献
扫码关注公众号
