重组人肝细胞生长因子 /Recombinant HGF, Human, HEK293,Animal-Free
产品编号 优惠促销 Alternate Text 规格 价格 货期
CM192-5HP 买一赠一 5 ug ¥600.00 现货
CM192-20HP 买一赠一 20 ug ¥1500.00 现货
CM192-100HP 买一赠一 100 ug ¥4500.00 现货
CM192-500HP 买一赠一 500 ug ¥13000.00 请询
CM192-1000HP 买一赠一 1 mg ¥20000.00 请询
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产品简介
背景描述 Hepatocyte Growth Factor (HGF) activates c-Met receptor's tyrosine kinase cascade, influencing cell growth and motility. Originating from mesenchymal cells, HGF is a multifunctional cytokine that plays a central role in angiogenesis, tumorigenesis, and tissue regeneration. Initially secreted as an inactive polypeptide, HGF undergoes cleavage to become an active heterodimeric molecule. The gene exhibits alternative splicing, resulting in diverse isoforms.
别称 Hepatocyte growth factor, Hepatopoietin-A, Scatter factor (SF), HPTA
蛋白编码 P14210
分子量 The protein has a calculated MW of 80.48 kDa. The protein migrates as 75-100 kDa under reducing condition (SDS-PAGE analysis).
表达系统 HEK293 cell
纯度 >90% as determined by SDS-PAGE analysis.
生物活性 Testing in process
内毒素检测 <1 EU per 1 μg of the protein by the LAL method.
蛋白序列 A DNA sequence encoding Human HGF Protein (#P14210)(Gln 32-Ser 728) was expressed with polyhistidine tag at the C-terminus.
蛋白标签 His Tag (C-term)
产品形式 The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
产品应用 Cell Culture
产品储存/运输
储存 Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输 Blue Ice
产品使用
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
实验数据展示
SDS- PAGE analysis of recombinant human HGF
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