cytokine

重组人神经调节蛋白beta 1 /Recombinant Heregulin Beta 1，Human，Animal-Free

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产品编号	优惠促销	规格	价格	货期
CM187-5HP	买一赠一	5 ug	￥600.00	现货
CM187-20HP	买一赠一	20 ug	￥1500.00	现货
CM187-100HP	买一赠一	100 ug	￥4500.00	现货
CM187-500HP	买一赠一	500 ug	￥13000.00	请询
CM187-1000HP	买一赠一	1 mg	￥20000.00	请询

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产品简介

背景描述	Neuregulin-1 (NRG-1, also called heuregulin1 or neu differentiation factor) is a glycoprotein that belongs to the neuregulins family. Structurally, Neuregulin-1 harbors tissue-speciﬁc N terminal sequence, followed by immunoglobulin-like (Ig-like) domains, an EGF-like domain, a transmembrane domain, and a cytoplasmic domain. NRG1 has multiple isoforms produced by alternative splicing. Heregulin- β1 (HRG- β1) is one of the isoforms, has been reported to engage the development and survival of cardiomyocytes derived from embryonic stem (ES) cells via activating MAPK-ERK and PI3K-AKT pathways. Moreover, HRG- β1 plays a central role in promoting the proliferation of neuronal progenitors from embryonic neural stem cells.
别称	NRG1 Beta 1, Heregulin-β1
蛋白编码	Q02297-6
分子量	The protein has a calculated MW of 8.42 kDa. The protein migrates as 8 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>95% as determined by SDS-PAGE analysis.
生物活性	Measure by its ability to induce MCF-7 cells proliferation. The ED₅₀ for this effect is < 10 ng/mL.
内毒素检测	<1 EU per 1 μg of the protein by the LAL method.
蛋白序列	A DNA sequence encoding Human Heregulin Beta 1 Protein (#Q02297-6)(Ser177-Glu241) was expressed with polyhistidine tag at the C-terminus.
蛋白标签	His Tag (C-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示

SDS- PAGE analysis of recombinant human Heregulin Beta 1

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