无动物源成分重组细胞因子

重组猪血管内皮生长因子 /Recombinant VEGF，Swine，Animal-Free

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产品编号	优惠促销	规格	价格	货期
CM020-5SP	买一赠一	5 ug	￥1200.00	现货
CM020-20SP		20 ug	￥3000.00	现货

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产品简介

背景描述	Swine Vascular endothelial Growth Factors (VEGF) is a protein that stimulate vasculogenesis and angiogenesis. SwineVEGF containing 165 residues with polyhistidine tag at the C-terminus. Swine VEGF is proteins involevd in embryonic development, new blood vessels repairing, and new vessels (collateral circulation) bypassing blocked vessels.
别称	vascular endothelial growth factor, VEGFA
蛋白编码	P49151
分子量	The protein has a calculated MW of 20.16 kDa. The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>95% as determined by SDS-PAGE.
生物活性	Testing in process
内毒素检测	<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列	MAPMAEGDQKPHEVVKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEEFNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR with polyhistidine tag at the C-terminus.
蛋白标签	His Tag (C-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示

SDS- PAGE analysis of recombinant swine VEGF

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