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重组小鼠核因子κ B受体活化因子配体 /Recombinant RANKL,Mouse,Animal-Free
产品编号 优惠促销 Alternate Text 规格 价格 货期
CM045-5MP 买大送小 5 μg ¥600.00 现货
CM045-20MP 买大送小 20 μg ¥1500.00 现货
CM045-100MP 买大送小 100 μg ¥3980.00 现货
CM045-500MP 买大送小 500 μg ¥9980.00 请询
CM045-1000MP 买大送小 1 mg ¥16980.00 请询
产品简介
背景描述 Receptor activator of NF-κB (RANK) ligand (RANKL) is type II transmembrane protein with an extracellular domain at the carboxy terminus of TNF cytokine superfamily. RANKL is a 19.8 kDa protein containing 317 residues and high expressed in T cells and T cell rich organs, such as thymus and lymph nodes. RANKL-RANK (RANKL receptor) plays an important role in bone metabolism, dysregulation, and immune system. RANKL deficiencies in mice or humans are associated with abnormally increase bone density and blemish in lymphoid organogenesis.
别称 receptor activator of nuclear factor kappa-Β ligand, soluble Receptor Activator of NF-kB Ligand, TNFSF11, TRANCE (TNF-Related Activation-induced Cytokine), OPGL, ODF (Osteoclast Differentiation Factor), CD254,sRNAK Ligand
蛋白编码 O35235
分子量 The protein has a calculated MW of 20.35 kDa. The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
表达系统 Escherichia coli
纯度 >98% as determined by SDS-PAGE.
生物活性 Measure by its ability to induce osteoclast differentiation in RAW264.7 cells. The ED₅₀ for this effect is <2 ng/mL.
内毒素检测 <0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列 MPAMMEGSWLDVAQRGKPEAQPFAHLTINAASIPSGSHKVTLSSWYHDRGWAKISNMTLSNGKLRVNQDGFYYLYANICFRHHETSGSVPTDYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGNSEFHFYSINVGGFFKLRAGEEISIQVSNPSLLDPDQDATYFGAFKVQDID with polyhistidine tag at the C-terminus.
蛋白标签 His Tag (C-term)
产品形式 The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
产品应用 Cell Culture
产品使用

1. Before opening, centrifuge at 3000 rpm for 5 mins.


2.Initial Reconstitution

· Reconstitute the Lyophilized Protein in sterile H₂O to a concentration of 100-200 μg/mL.

· Then, incubate it at room temperature for at least 20 mins to ensure sufficient dissolution.

   Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

· Store at 2°C to 8°C for up to 1 week.


3.Extended Storage

· After the initial reconstitution, further dilute the reconstituted protein in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/ml.

· Prepare aliquots (≥20 μl).

· Store at -20°C or -80°C for 3 to 6 months.

   Avoid repeated freeze-thaw cycles.

产品储存/运输
储存

Lyophilized Protein: Store at -20°C for 1 year.

Initial Reconstitution: Store at 2°C to 8°C for up to 1 week.

Extended Storage: Store at -20°C or -80°C for 3 to 6 months with a carrier protein or stabilizer.

运输 Blue Ice
实验数据展示
SDS-PAGE analysis of recombinant mouse RANKL
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