| 背景描述 | Interleukin 33 (IL-33) is a 17.65 kDa cytokine with 159 amino acid residues. IL-33 is a member of the IL-1 family and secreted from various cell types, such as mast cells, macrophages, endothelial cells, and epithelial cells. IL-33 activates NF-κB and MAPK signaling pathways that stimulate the secretion of type 2 cytokines like IL-5 and IL-13 when IL-33 binds to the ST2 receptor primarily expressed in mast cells and Th2 cells. In addition to Th type 2 responses, IL-33 participates in maintaining barrier tissue defense. |
| 别称 | interleukin 33, IL-1 F11, NF-HEV, 9230117N10Rik, IL33 |
| 蛋白编码 | Q8BVZ5 |
| 分子量 | The protein has a calculated MW of 18.51 kDa. The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis). |
| 表达系统 | Escherichia coli |
| 纯度 | >98% as determined by SDS-PAGE. |
| 生物活性 | Measure by its ability to induce proliferation in D10.G4.1 cells. The ED₅₀ for this effect is <40 pg/mL. The specific activity of recombinant mouse IL-33 is > 2 x 10⁷ IU/mg. |
| 内毒素检测 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 蛋白序列 | MSIQGTSLLTQSPASLSTYNDQSVSFVLENGCYVINVDDSGKDQEQDQVLLRYYESPCPASQSGDGVDGKKLMVNMSPIKDTDIWLHANDKDYSVELQRGDVSPPEQAFFVLHKKSSDFVSFECKNLPGTYIGVKDNQLALVEEKDESCNNIMFKLSKI with polyhistidine tag at the C-terminus. |
| 蛋白标签 | His Tag (C-term) |
| 产品形式 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us. |
| 产品应用 | Cell Culture |
1. Before opening, centrifuge at 3000 rpm for 5 mins.
2.Initial Reconstitution
· Reconstitute the Lyophilized Protein in sterile H₂O to a concentration of 100-200 μg/mL.
· Then, incubate it at room temperature for at least 20 mins to ensure sufficient dissolution.
Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
· Store at 2°C to 8°C for up to 1 week.
3.Extended Storage
· After the initial reconstitution, further dilute the reconstituted protein in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/ml.
· Prepare aliquots (≥20 μl).
· Store at -20°C or -80°C for 3 to 6 months.
Avoid repeated freeze-thaw cycles.
| 储存 | Lyophilized Protein: Store at -20°C for 1 year. Initial Reconstitution: Store at 2°C to 8°C for up to 1 week. Extended Storage: Store at -20°C or -80°C for 3 to 6 months with a carrier protein or stabilizer. |
| 运输 | Blue Ice |
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| SDS-PAGE analysis of recombinant mouse IL-33 |
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