重组小鼠白细胞介素22 /Recombinant IL-22,Mouse,Animal-Free
产品编号 优惠促销 Alternate Text 规格 价格 货期
CM019-5MP 买一赠一 5 ug ¥600.00 现货
CM019-20MP 20 ug ¥1500.00 现货
CM019-100MP 100 ug ¥4500.00 现货
CM019-500MP 500 ug ¥13000.00 请询
CM019-1000MP 1 mg ¥20000.00 请询
在线咨询
产品简介
背景描述 Interleukin 22 (IL-22) is an α-helical cytokine, predicts a molecular mass of 16.9 kDa. It is produced by T-helper (Th)-17 cells, γδ T cells, NKT cells and newly described innate lymphoid cells (ILCs). Effects involve stimulation of cell survival, proliferation and synthesis of antimicrobials including S110, Reg3β, Reg3γ and defensins.
别称 interleukin 22, IL-TIF, Cytokine ZCYTO18, If2b1, ILTIFa, IL22
蛋白编码 Q9JJY9
分子量 The protein has a calculated MW of 17.58 kDa. The protein migrates about 17 kDa under reducing condition (SDS-PAGE analysis).
表达系统 Escherichia coli
纯度 >98% as determined by SDS-PAGE.
生物活性 Measure by its ability to induce IL-10 secretion in COLO205 cells. The ED₅₀ for this effect is <0.3 ng/mL.
内毒素检测 <0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列 MLPVNTRCKLEVSNFQQPYIVNRTFMLAKEASLADNNTDVRLIGEKLFRGVSAKDQCYLMKQVLNFTLEDVLLPQSDRFQPYMQEVVPFLTKLSNQLSSCHISGDDQNIQKNVRRLKETVKKLGESGEIKAIGELDLLFMSLRNACV with polyhistidine tag at the C-terminus.
蛋白标签 His Tag (C-term)
产品形式 The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
产品应用 Cell Culture
产品使用
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
产品储存/运输
储存 Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输 Blue Ice
实验数据展示
SDS- PAGE analysis of recombinant mouse IL-22
文献引用
暂无参考文献
相关产品