无动物源成分重组细胞因子

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重组小鼠成纤维生长因子-2/碱性成纤维细胞生长因子 /Recombinant FGF-2(bFGF)，Mouse，Animal-Free

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产品编号	优惠促销	规格	价格	货期
CM058-20MP	买一赠一	20 ug	￥600.00	现货
CM058-100MP	买一赠一	100 ug	￥1500.00	现货
CM058-500MP	买一赠一	500 ug	￥4500.00	现货
CM058-1000MP	买一赠一	1 mg	￥7000.00	请询

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产品简介

背景描述	Basic fibroblast Growth Factors (FGF-2, bFGF), a pleiotropic cytokine, plays multiple roles in different cells and tissues. FGF-2 can stimulate smooth muscle cell growth, wound healing, and tissue repair. In addition, FGF-2 has been shown to regulate the generation of neurons and astrocytes from progenitor cells. FGF-2 are also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth, and invasion. As a multifunctional cytokine, FGF-2 is first isolated from the pituitary. Later, it was identified from various cell types including cardiac myocytes, cardiac fibroblasts, endothelial cells, and smooth muscle cells.
别称	fibroblast growth factor basic, HBGF-2, Prostatropin, bFGF, FGF basic
蛋白编码	P15655(Mouse), P13109.1(Rat)
分子量	The protein has a calculated MW of 17.2 kDa. The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>98% as determined by SDS-PAGE.
生物活性	Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <1.5 ng/mL. The specific activity of recombinant mouse FGF-2 is approximately >1 x 10⁶ IU/mg.
内毒素检测	<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列	ALPEDGGAAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHVKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTEECFFFERLESNNYNTYRSRKYSSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS with polyhistidine tag at the N-terminus.
蛋白标签	His Tag (N-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 0.01% sarkosyl in 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示

SDS- PAGE analysis of recombinant mouse FGF-2

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