无动物源成分重组细胞因子

重组小鼠CD27配体 /Recombinant CD27L，Mouse，Animal-Free

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产品编号	优惠促销	规格	价格	货期
CM089-5MP	买一赠一	5 ug	￥600.00	现货
CM089-20MP		20 ug	￥1500.00	现货
CM089-100MP		100 ug	￥4500.00	现货
CM089-500MP		500 ug	￥13000.00	请询
CM089-1000MP		1 mg	￥20000.00	请询

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产品简介

背景描述	CD27L is also named for CD70 which is one member of TNF family. CD27L is expressed on T and B lymphocytes and mature DCs. It binds the CD27, which is on the antigen-presenting cells. CD27L is a 19.2 kDa cytokine with 148 amino acid residues which is a transmembrane glycoprotein. CD27L plays an important role in the regulation of T cell proliferation which is also a good target of cancer immunotherapy.
别称	CD27 ligand, soluble CD27 Ligand, sCD27 Ligand, TNFSF7, CD70, Tnfs, Tnlg8a
蛋白编码	O55237
分子量	The protein has a calculated MW of 17.25 kDa. The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>98% as determined by SDS-PAGE.
生物活性	Measure by its ability to induce proliferation in mouse T cells in the presence of the anti-CD3 antibody. The ED₅₀ for this effect is <4.5 μg/mL.
内毒素检测	<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列	QQQRLLEHPEPHTAELQLNLTVPRKDPTLRWGAGPALGRSFTHGPELEEGHLRIHQDGLYRLHIQVTLANCSSPGSTLQHRATLAVGICSPAAHGISLLRGRFGQDCTVALQRLTYLVHGDVLCTNLTLPLLPSRNADETFFGVQWICP with polyhistidine tag at the N-terminus.
蛋白标签	His Tag (N-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示

SDS- PAGE analysis of recombinant mouse CD27L

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