无动物源成分重组细胞因子

重组人肿瘤坏死因子β /Recombinant TNF beta，Human，Animal-Free

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产品编号	优惠促销	规格	价格	货期
CM047-5HP	买一赠一	5 ug	￥600.00	现货
CM047-20HP	买一赠一	20 ug	￥1500.00	现货
CM047-100HP		100 ug	￥4500.00	现货
CM047-500HP		500 ug	￥13000.00	请询
CM047-1000HP		1 mg	￥20000.00	请询

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产品简介

背景描述	Human TNF beta (Lymphotoxin-alpha) is a 22 kDa cytokine with 205 amino acid residues. TNF beta belongs to tumor necrosis factor (TNF) superfamily as TNF alpha. Binding to lymphotoxin-β receptor (LtβR), canonical and non-canonical NF-κB signal pathway will be activated. TNF beta is related to apoptosis and inflammatory signals
别称	tumor necrosis factor beta, TNFSF1B, Lymphotoxin-alpha (LT-α),LTA
蛋白编码	P01374
分子量	The protein has a calculated MW of 18.65 kDa. The protein migrates as 21 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>98% as determined by SDS-PAGE.
生物活性	Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED₅₀ for this effect is <3 pg/mL. The specific activity of recombinant human TNF beta is > 3.3x 10⁸ IU/mg.
内毒素检测	<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列	LPGVGLTPSAAQTARQHPKMHLAHSTLKPAAHLIGDPSKQNSLLWRANTDRAFLQDGFSLSNNSLLVPTSGIYFVYSQVVFSGKAYSPKATSSPLYLAHEVQLFSSQYPFHVPLLSSQKMVYPGLQEPWLHSMYHGAAFQLTQGDQLSTHTDGIPHLVLSPSTVFFGAFAL with polyhistidine tag at the N-terminus.
蛋白标签	His Tag (N-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示

SDS- PAGE analysis of recombinant human TNF beta

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