无动物源成分重组细胞因子

重组人肿瘤坏死因子超家族成员14 /Recombinant LIGHT，Human，Animal-Free

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产品编号	优惠促销	规格	价格	货期
CM057-5HP	买一赠一	5 ug	￥600.00	现货
CM057-20HP		20 ug	￥1500.00	现货
CM057-100HP		100 ug	￥4500.00	现货
CM057-500HP		500 ug	￥13000.00	请询
CM057-1000HP		1 mg	￥20000.00	请询

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产品简介

背景描述	LIGHT, also known as TNFSF14 is a member of the TNF superfamily that produced by multiple immune cells such as activated T cells and immature dendritic cells (DCs). LIGHT is a 29 kDa type II transmembrane protein, which serves as a ligand for both lymphotoxin β receptor (LTβR) and TNFSF signaling receptors (TNFRSF14/HVEM). Besides, LIGHT can amplify NF-kB signaling pathway in T cells when presenting anti-CD3 antibody treatment. Additionally, LIGHT can stimulate T cell proliferation and IFN-expression via interacting with TNFRS13 /HVEM.
别称	TNFSF14, HVEM-L, CD258, LTg
蛋白编码	O43557.2
分子量	The protein has a calculated MW of 20.30 kDa. The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>98% as determined by SDS-PAGE.
生物活性	Measure by its ability to induce cytotoxicity in HT-29 cells in the presence of IFN-gamma. The ED₅₀ for this effect is < 10 ng/mL.
内毒素检测	<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列	MRLGEMVTRLPDGPAGSWEQLIQERRSHEVNPAAHLTGANSSLTGSGGPLLWETQLGLAFLRGLSYHDGALVVTKAGYYYIYSKVQLGGVGCPLGLASTITHGLYKRTPRYPEELELLVSQQSPCGRATSSSRVWWDSSFLGGVVHLEAGEKVVVRVLDERLVRLRDGTRSYFGAFMV with polyhistidine tag at the C-terminus.
蛋白标签	His Tag (C-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示

SDS- PAGE analysis of recombinant human LIGHT

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