无动物源成分重组细胞因子

重组人（哺乳动物细胞表达）高迁移率族蛋白B1 /Recombinant HMGB1 C23AC45A，Human，Animal-Free

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产品编号	优惠促销	规格	价格	货期
CM176-5HP		5 ug	￥600.00	现货
CM176-20HP		20 ug	￥1500.00	现货
CM176-100HP		100 ug	￥4500.00	现货
CM176-500HP		500 ug	￥13000.00	请询
CM176-1000HP		1 mg	￥20000.00	请询

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产品简介

背景描述	High Mobility Group protein B1 protein (HMGB1) is the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 25 kDa single chain polypeptide containing three domains: two N-terminal HMG boxes A and B, and a negatively charged 30 a.a. C-terminal region that contains only Asp and Glu. Post-translational modification on HMGB1 have been reported, affects its localization, receptor interactions, and function. HMGB1, with a disulfide bond between C23 and C45 , have been reported that cause cytokine production and the activation of NF-κB. Therefore, we developed the HMGB1 C23A& C45A mutant proteins, eliminant the disulfide bond formation.
别称	high mobility group box 1, HMG-1, HMG1, HMG3, SBP-1
蛋白编码	P09429
分子量	The protein has a calculated MW of 36.36 kDa. The protein migrates as 35-48 kDa under reducing condition (SDS-PAGE analysis).
表达系统	HEK293
纯度	>98% as determined by SDS-PAGE.
生物活性	Measure by its ability to induce TNF alpha in RAW264.7 cells. The ED₅₀ for this effect is <10 μg/mL.
内毒素检测	<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列	MGKGDPKKPRGKMSSYAFFVQTAREEHKKKHPDASVNFSEFSKKASERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEDEEEDDDDE with polyhistidine-SUMO tag at the N-terminus
蛋白标签	His-SUMO Tag (N-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示

SDS- PAGE analysis of recombinant human HMGB1 C23AC45A

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