重组人肝癌源性生长因子 /Recombinant HDGF,Human,Animal-Free
产品编号 优惠促销 Alternate Text 规格 价格 货期
CM122-5HP 买一赠一 5 ug ¥600.00 现货
CM122-20HP 20 ug ¥1500.00 现货
CM122-100HP 100 ug ¥4500.00 现货
CM122-500HP 500 ug ¥13000.00 请询
CM122-1000HP 1 mg ¥20000.00 请询
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产品简介
背景描述 Heparin binding Growth Factors (HDGF) is a 27.60 kDa hepatoma-derived Growth Factors with 246 amino acid residues. HDGF is expressed from mitochondria and proteasome. Functionally, it is a potent mitogen, stimulating the growth of vascular smooth muscle cells, hepatoma cells and endothelial cells, acting as a transcriptional repressor.
别称 hepatoma-derived growth factor, HMG1L2
蛋白编码 P51858
分子量 The protein has a calculated MW of 27.60 kDa. The protein migrates as 45 kDa under reducing condition (SDS-PAGE analysis).
表达系统 Escherichia coli
纯度 >98% as determined by SDS-PAGE.
生物活性 Testing in process
内毒素检测 <0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列 MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL with polyhistidine tag at the C-terminus.
蛋白标签 His Tag (C-term)
产品形式 The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
产品应用 Cell Culture
产品储存/运输
储存 Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输 Blue Ice
产品使用
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
实验数据展示
SDS- PAGE analysis of recombinant human HDGF
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