无动物源成分重组细胞因子

重组人粒细胞集落刺激因子 /Recombinant G-CSF，Human，Animal-Free

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产品编号	优惠促销	规格	价格	货期
CM117-5HP	买一赠一	5 ug	￥600.00	现货
CM117-20HP	买一赠一	20 ug	￥1500.00	现货
CM117-100HP	买一赠一	100 ug	￥4500.00	现货
CM117-500HP	买一赠一	500 ug	￥13000.00	请询
CM117-1000HP	买一赠一	1 mg	￥20000.00	请询

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产品简介

背景描述	Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins and makes the bone marrow produce more white blood cells so it can reduce the risk of infection after some types of cancer treatment also is an established useful clinical agent for increasing neutrophilic granulocytes levels. G-CSF is a 18.67 kDa protein having 207 amino acid which secreted by monocytes, macrophages, fibroblasts, and endothelial cells, regulates cell growth, maturation, development of myeloid cells.
别称	granulocyte colony-stimulating factor, CSF-3, MGI-1G, pluripoietin, CSF3
蛋白编码	NP_757373
分子量	The protein has a calculated MW of 19.48 kDa. The protein migrates as 21kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>98% as determined by SDS-PAGE.
生物活性	Measure by its ability to induce proliferation in NFS-60 cells. The ED₅₀ for this effect is <0.5 ng/mL.
内毒素检测	<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列	TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP with polyhistidine tag at the N-terminus.
蛋白标签	His Tag (N-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示


SDS- PAGE analysis of recombinant human G-CSF	Human G-CSF, His Tag, E. coli induced NFS-60 cell proliferation, with the ED₅₀ at 44 pg/mL.

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