| 背景描述 | Fibroblast Growth Factors-9 (FGF-9) is a 23.4 kDa member of the fibroblast Growth Factors with 208 amino acid residues. FGF-9 is an important role embryonic development, cell proliferation, cell differentiation and cell migration in cell functions. It can regulate bone development, glial cell growth and differentiation during development, angiogenesis, differentiation and survival of neuronal cells. |
| 别称 | fibroblast growth factor 9, GAF (Glia-Activating Factor), HBGF-9, FGF9 |
| 蛋白编码 | P31371 |
| 表达系统 | Escherichia coli |
| 纯度 | >95% as determined by SDS-PAGE. |
| 生物活性 | Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <2 ng/mL. |
| 内毒素检测 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 蛋白序列 | MPLGEVGNYFGVQDAVPFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS with polyhistidine tag at the C-terminus. |
| 蛋白标签 | His Tag (C-term) |
| 产品形式 | The protein was lyophilized from a 0.2 µm filtered solution containing 50 mM Tris, 150 mM NaCl, pH 8.5. If you have any concerns or special requirements, please confirm with us. |
| 产品应用 | Cell Culture |
| 分子量 | The protein has a calculated MW of 22.14 kDa. The protein migrates as 24 kDa under reducing condition (SDS-PAGE analysis). |
1. Before opening, centrifuge at 3000 rpm for 5 mins.
2. Initial Reconstitution
· Reconstitute the Lyophilized Protein in sterile H₂O to a concentration of 100-200 μg/mL.
· Then, incubate it at room temperature for at least 20 mins to ensure sufficient dissolution.
Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
· Store at 2°C to 8°C for up to 1 week.
3. Extended Storage
· After the initial reconstitution, further dilute the reconstituted protein in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/mL.
· Prepare aliquots (≥20 μL).
· Store at -20°C or -80°C for 3 to 6 months.
Avoid repeated freeze-thaw cycles.
| 储存 | Lyophilized Protein: Store at -20°C for 1 year. Initial Reconstitution: Store at 2°C to 8°C for up to 1 week. Extended Storage: Store at -20°C or -80°C for 3 to 6 months with a carrier protein or stabilizer. |
| 运输 | Blue Ice |
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| SDS-PAGE analysis of recombinant human FGF-9 |
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