| 背景描述 | Fibroblast Growth Factors-8b (FGF-8b) is a 21.3 kDa member of the fibroblast Growth Factors with 183 amino acid residues. FGF-8 is an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. In embryogenesis function, FGF8 is necessary in limb, brain, eye and ear formation. FGF-8b having a more potent inductive activity due to higher affinity for Fgf receptors. |
| 别称 | fibroblast growth factor 8b, AIGF, HBGF-8, FGF8 |
| 蛋白编码 | P55075-3 |
| 表达系统 | Escherichia coli |
| 纯度 | >95% as determined by SDS-PAGE. |
| 生物活性 | Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is 1.4-3.8 ng/mL. The specific activity of recombinant human FGF-8b is > 2 x 10⁵ IU/mg. |
| 内毒素检测 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 蛋白序列 | A DNA sequence encoding Human FGF-8b Protein(#P55075-3) (Gln23-Arg215) was expressed with polyhistidine tag at the C-terminus. |
| 蛋白标签 | His Tag (C-term) |
| 产品形式 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us. |
| 产品应用 | Cell Culture |
| 分子量 | The protein has a calculated MW of 22.14 kDa. The protein migrates as 23 kDa under reducing condition (SDS-PAGE analysis). |
1. Before opening, centrifuge at 3000 rpm for 5 mins.
2. Initial Reconstitution
· Reconstitute the Lyophilized Protein in sterile H₂O to a concentration of 100-200 μg/mL.
· Then, incubate it at room temperature for at least 20 mins to ensure sufficient dissolution.
Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
· Store at 2°C to 8°C for up to 1 week.
3. Extended Storage
· After the initial reconstitution, further dilute the reconstituted protein in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/ml.
· Prepare aliquots (≥20 μl).
· Store at -20°C or -80°C for 3 to 6 months.
Avoid repeated freeze-thaw cycles.
| 储存 | Lyophilized Protein: Store at -20°C for 1 year. Initial Reconstitution: Store at 2°C to 8°C for up to 1 week. Extended Storage: Store at -20°C or -80°C for 3 to 6 months with a carrier protein or stabilizer. |
| 运输 | Blue Ice |
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| SDS-PAGE analysis of recombinant human FGF-8b | Human FGF-8b, His Tag, E. coli induced 3T3 cell proliferation, with the ED₅₀ at 3.51 ng/mL. |
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