无动物源成分重组细胞因子

重组人成纤维细胞生长因子-11亚型1 /Recombinant FGF-11 isoform 1，Human，Animal-Free

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产品编号	优惠促销	规格	价格	货期
CM100-5HP	买一赠一	5 ug	￥600.00	现货
CM100-20HP		20 ug	￥1500.00	现货
CM100-100HP		100 ug	￥4500.00	现货
CM100-500HP		500 ug	￥13000.00	请询
CM100-1000HP		1 mg	￥20000.00	请询

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产品简介

背景描述	The Fibroblast Growth Factors (FGF) family has two group, secreted FGFs and intracellular FGFs (iFGFs). iFGFs are related to voltage-gated sodium (Nav) channels. Human FGF-11 isoform 1 is a 25 kDa cytokine with 225 amino acid residues.
别称	fibroblast growth factor 11 isoform 1, FHF-3, FHF3, FGF11
蛋白编码	Q92914
分子量	The protein has a calculated MW of 25.81 kDa. The protein migrates as 30 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>98% as determined by SDS-PAGE.
生物活性	Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <0.2 ng/mL.
内毒素检测	<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列	MAALASSLIRQKREVREPGGSRPVSAQRRVCPRGTKSLCQKQLLILLSKVRLCGGRPARPDRGPEPQLKGIVTKLFCRQGFYLQANPDGSIQGTPEDTSSFTHFNLIPVGLRVVTIQSAKLGHYMAMNAEGLLYSSPHFTAECRFKECVFENYYVLYASALYRQRRSGRAWYLGLDKEGQVMKGNRVKKTKAAAHFLPKLLEVAMYQEPSLHSVPEASPSSPPAP with polyhistidine tag at the C-terminus.
蛋白标签	His Tag (C-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示


SDS- PAGE analysis of recombinant human FGF-11 isoform 1	Human FGF-11 isoform 1, His Tag, E. coli induced 3T3 cell proliferation, with the ED₅₀ at 0.17 ng/mL.

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