无动物源成分重组细胞因子

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重组人表皮生长因子 /Recombinant EGF，Human，Animal-Free

产品说明书

产品编号	优惠促销	规格	价格	货期
CM120-100HP	买一赠一	100 ug	￥600.00	现货
CM120-500HP	买一赠一	500 ug	￥1500.00	现货
CM120-1000HP	买一赠一	1 mg	￥2100.00	现货

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产品简介

背景描述	Human epidermal Growth Factors (EGF) is a 6 kDa cytokine with 53 amino acid residues. EGF is mainly secreted from ectodermal cells, monocytes, kidney and duodenal glands. Upon binding to its receptor, EGFR, EGF acts to stimulate cell growth and proliferation of epithelial cells, play important roles in many developmental processes including accelerate tooth eruption, inhibits gastric acid secretion, and involve in wound healing.
别称	epidermal growth factor, Urogastrone, URG
蛋白编码	P01133
分子量	The protein has a calculated MW of 7.16 kDa. The protein migrates as 9-11 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>95% as determined by SDS-PAGE and SEC-HPLC.
生物活性	Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is < 1.0 ng/mL. The specific activity of recombinant human EGF is approximately >1.0 x 10⁶ IU/mg.
内毒素检测	<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列	MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR with polyhistidine tag at the C-terminus
蛋白标签	His Tag (C-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示


SDS-PAGE analysis of recombinant human EGF	The purity of recombinant human EGF is greater than 95% as determined by SEC-HPLC.

文献引用

USP8 positively regulates hepatocellular carcinoma tumorigenesis and confers ferroptosis resistance through β-catenin stabilization. Cell Death Dis. 2023 Jun 13;14(6):360. (IF 9.696) (Human EGF/bFGF)