无动物源成分重组细胞因子

重组人纤毛神经营养因子 /Recombinant CNTF，Human，Animal-Free

说明书下载

产品编号	优惠促销	规格	价格	货期
CM137-5HP	买一赠一	5 ug	￥600.00	现货
CM137-20HP		20 ug	￥1500.00	现货
CM137-100HP		100 ug	￥4500.00	现货
CM137-500HP		500 ug	￥13000.00	请询
CM137-1000HP		1 mg	￥20000.00	请询

在线咨询

产品简介

背景描述	Ciliary neurotrophic factor (CNTF) is the member of IL-6 cytokine family and expressed in the nervous system. CNTF is 22.9 kDa neurotrophic factor containing 200 residues, which shows multiple effects in vertebrate retinogenesis. Besides, CNTF acts as a promoter that not only accelerates adult neurogenesis but also increases the survival of neuron after injury. On the other hands, reducing number of hippocampal GABAergic interneurons, as well as 5-HT levels and 5-HT1A receptor expression has been found in CNTF knockout female mice, indicating that CNTF may influence affective behavior in females by regulation of neurotransmission.
别称	ciliary neurotrophic factor, HCNTF
蛋白编码	P26441
分子量	The protein has a calculated MW of 23.74 kDa. The protein migrates as 25 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>98% as determined by SDS-PAGE.
生物活性	Measure by its ability to induce proliferation in TF-1 cells. The ED₅₀ for this effect is <0.15 μg/mL.
内毒素检测	<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列	MAFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTESYVKHQGLNKNINLDSADGMPVASTDQWSELTEAERLQENLQAYRTFHVLLARLLEDQQVHFTPTEGDFHQAIHTLLLQVAAFAYQIEELMILLEYKIPRNEADGMPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRFISSHQTGIPARGSHYIANNKKM with polyhistidine tag at the C-terminus
蛋白标签	His Tag (C-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示

SDS- PAGE analysis of recombinant human CNTF

文献引用

暂无参考文献