无动物源成分重组细胞因子

重组人骨形态发生蛋白-14 /Recombinant BMP-14，Human，Animal-Free

说明书下载

产品编号	优惠促销	规格	价格	货期
CM076-5HP	买一赠一	5 ug	￥600.00	现货
CM076-20HP		20 ug	￥1500.00	现货
CM076-100HP		100 ug	￥4500.00	现货
CM076-500HP		500 ug	￥13000.00	请询
CM076-1000HP		1 mg	￥20000.00	请询

在线咨询

产品简介

背景描述	Bone Morphogenetic Protein-14 (BMP-14), known as Growth differentiation factor 5 (GDF5), is an extracellular multifunctional cytokine that is also a member of the TGFβ family. BMP-14 can bind with the TGFβ receptor and trigger SMAD protein signal transduction. BMP-14 plays a role in skeletal and joint development and increases the survival of neurons that respond to the neurotransmitter dopamine.
别称	bone morphogenetic protein 14, Cartilage-Derived Morphogenetic Protein-1,Growth/Differentiation Factor-5,GDF-5, CDMP-1, BMP14, GDF5, CDMP1
蛋白编码	P43026
分子量	The protein has a calculated MW of 14.52 kDa. The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>98% as determined by SDS-PAGE.
生物活性	Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <14 ng/mL.
内毒素检测	<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列	MAPLATRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIAPLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESCGCR with polyhistidine tag at the C-terminus.
蛋白标签	His Tag (C-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示


SDS- PAGE analysis of recombinant human BMP-14	Human BMP-14, His Tag, E. coli induced alkaline phosphatase production by ATDC5 cells, with the ED₅₀ at 13.52 ng/mL.

文献引用

暂无参考文献