无动物源成分重组细胞因子

重组人骨形态发生蛋白-11 /Recombinant BMP-11，Human，Animal-Free

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产品编号	优惠促销	规格	价格	货期
CM073-5HP	买一赠一	5 ug	￥600.00	现货
CM073-20HP		20 ug	￥1500.00	现货
CM073-100HP		100 ug	￥4500.00	现货
CM073-500HP		500 ug	￥13000.00	请询
CM073-1000HP		1 mg	￥20000.00	请询

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产品简介

背景描述	Bone Morphogenetic Protein-11 (BMP-11), known as Growth differentiation factor 11 (GDF11), is an extracellular multifunctional signaling cytokine that is also a member of the TGFβ family. BMP-11 can bind with the TGFβ receptor and is involved in SMAD protein signal transduction. Moreover, it promotes the formation of blood vessels and nerves that can control anterior-posterior patterning.
别称	bone morphogenetic protein 11, Growth/Differentiation Factor-11, GDF-11, BMP11
蛋白编码	O95390
分子量	The protein has a calculated MW of 13.40 kDa. The protein migrates as 16 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>98% as determined by SDS-PAGE.
生物活性	Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <11 ng/mL. Measure by its ability to induce hemoglobin expression in K562 cells. The ED₅₀ for this effect is <4 ng/mL.
内毒素检测	<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列	MNLGLDCDEHSSESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGQCEYMFMQKYPHTHLVQQANPRGSAGPCCTPTKMSPINMLYFNDKQQIIYGKIPGMVVDRCGCS with polyhistidine tag at the C-terminus.
蛋白标签	His Tag (C-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl and 50 mM L-arginine, pH 4.5. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示


SDS- PAGE analysis of recombinant human BMP-11	Human BMP-11, His Tag, E. coli induced alkaline phosphatase production by ATDC5 cells, with the ED₅₀ at 10.93 ng/mL.

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