SARS-CoV-2 Trimeric Spike Protein (Delta B1.617.2 Variant), His Tag, HEK293
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Product Specifications
Background Coronaviruses (Delta B1.617.2 Variant) are enveloped positive-sense RNA viruses. Spike protein on the viral envelope and the cognate receptor on the surface of host cells that are essential for entry into host cells upon receptor binding and membrane fusion. Spike proteins are target for neutralization antibody, and mediate membrane fusion and virus entry.Trimeric spike protein is about 180 kDa, and contains two subunits, S1 and S2, mediating attachment and membrane fusion.
Synonyms Spike glycoprotein, S glycoprotein, E2, Peplomer protein
Uniprot ID P0DTC2
Molecular Weight The protein has a calculated MW of 133.12 kDa. The protein migrates above 180 kDa under reducing condition (SDS-PAGE analysis).
Expression System HEK293 cell
Purity >98% as determined by SDS-PAGE analysis.
Protein Sequence Ser13-Pro1213
Protein Tag His Tag (C-term)
Form Liquid. Phosphate buffered saline, pH 7.4.
Product Note
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at RT for at least 20 min to ensure sufficient re-dissolved.
Storage/Shipping
Stability & Storage Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Shipping Blue Ice
Scientific Data
No experimental data is available
Citations
No references are available
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