SARS-CoV-2 Spike Protein (RBD), His tag, E. coli

Catalog#	Promotion	Size	Price	Availability
CM007-100PCoV		5 μg	$563.00

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Product Specifications

Background	Coronaviruses are enveloped positive-sense RNA viruses. Spike protein on the viral envelope and the cognate receptor on the surface of host cells that are essential for entry into host cells upon receptor binding and membrane fusion. Spike proteins are target for neutralization antibody, and mediate membrane fusion and virus entry. Receptor-binding domain (RBD), which is the S protein mediates viral entry into host cells by first binding to a host receptor.
Synonyms	Spike glycoprotein, S glycoprotein, E2, Peplomer protein
Uniprot ID	P0DTC2
Molecular Weight	The protein has a calculated MW of 26.04 kDa. The protein migrates as 25-35 kDa under reducing condition (SDS-PAGE analysis).
Expression System	Escherichia coli
Purity	>98% as determined by SDS-PAGE analysis.
Protein Sequence	Arg319-Asn541
Protein Tag	His Tag (C-term)
Form	Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

Storage/Shipping

Stability & Storage	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Shipping	Blue Ice

Product Note

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at RT for at least 20 min to ensure sufficient re-dissolved.

Scientific Data

No experimental data is available

Citations

No references are available