| Description | AccuBlot™ Anti-Rabbit IgG (H+L) Antibody (HRP) is conjugated with high-activity HRP. In coimmunoprecipitation (co-IP) assay, denatured IgG from IP samples may show interference and result in immunoblotting at ~55 kDa (heavy chain) and ~25 kDa (light chain) by using traditional secondary antibodies. The products of AccuBlot™ series are suitable for native IgG detection. |
| Form | Liquid |
| Reactivity | Rabbit |
| Clonality | Monoclonal |
| Immunogen | Purified rabbit IgG |
| Concentration | Lot-dependent, please refer to product label. |
| Conjugation | HRP |
| Storage Buffer | Phosphate Buffered Saline containing 1% BSA, 0.03% ProClin 300 and 50% glycerol, pH 7.4. |
| Isotype | IgG2b |
Recommended dilution factor:
ELISA: 1:2500-10000
WB:1:1000-3000
Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
| Stability & Storage | This product is stable after storage at:2-8°C for 2 weeks under sterile conditions from date of receipt.-20°C or -80°C for 12 months under sterile conditions from date of receipt.Avoid repeated freeze/thaw cycles and protect from light.Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage. |
No experimental data is available
No references are available
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