Form | Liquid |
Description | Mouse monoclonal anti-V5 antibody is an antibody specific to GKPIPNPLLGLDST (V5) tags which can be applied to several immunoassays including ELISA, WB, IHC, and Flow cytometry. This antibody has been identified in several sources (bacteria, yeast, and mammalian cells) of V5-fusion protein. |
Reactivity | Recognizes tag sequence of GKPIPNPLLGLDST on either the N- or C-terminus of recombinant fusion protein. |
Clonality | Monoclonal, clone 7-1-8 |
Immunogen | Pepetide: GKPIPNPLLGLDST (V5) |
Concentration | 1 mg/mL |
Conjugation | Unconjugated |
Storage Buffer | Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4. |
Isotype | IgG2b |
Stability & Storage | This product is stable after storage at: 2-8°C for 2 weeks under sterile conditions from date of receipt. -20°C or -80°C for 12 months under sterile conditions from date of receipt. Avoid repeated freeze/thaw cycles. Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage. |
Recommended dilution factor:
ELISA: 1:5000-20000
WB:1:1000-5000
IP: 1:500-2000
IFA: 1:500-1000
FACS: 1:500-1000
Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.