cytokine

Recombinant TGF beta 3，Human，Animal-Free

Datasheet

Catalog#	Promotion	Size	Price	Availability
CM168-5HP		5 ug	$75.00
CM168-20HP		20 ug	$188.00
CM168-100HP		100 ug	$563.00
CM168-500HP		500 ug	$1625.00
CM168-1000HP		1 mg	$2500.00

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Product Specifications

Background	Transforming Growth Factors-Beta 3 (TGF-beta 3) is belonging to the TGF-β superfamily TGF-β3 is 86%, 91% similar to TGF-β1 and TGF-β2. TGF-beta 3 is a 12.8 kDa protein containing 412 amino acids, which could inhibitor of DNA synthesis, increases cellular proliferation, essential mediator of EMT in cardiac morphogenesis, and up-regulated by milk stasis and induces apoptosis in mammary gland epithelium during involution.
Synonyms	transforming growth factor beta 3, ARVD, ARVD1, LDS5, RNHF, TGFB3, TGF-B3
Uniprot ID	P10600
Molecular Weight	The protein has a calculated MW of 13.66 kDa. The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis).
Expression System	Escherichia coli
Purity	>98% as determined by SDS-PAGE.
Activity	Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells. The ED₅₀ for this effect is <50 pg/mL. The specific activity of recombinant human TGF beta 3 is > 2 x 10⁷ IU/mg.
Endotoxin Level	<0.1 EU per 1 μg of the protein by the LAL method.
Protein Sequence	MALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS with polyhistidine tag at the C-terminus.
Protein Tag	His Tag (C-term)
Form	The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5. If you have any concerns or special requirements, please confirm with us.
Application	Cell Culture

Storage/Shipping

Stability & Storage	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Shipping	Blue Ice

Product Note

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile 10 mM HCl to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Scientific Data


SDS- PAGE analysis of recombinant human TGF beta 3	Human TGF Beta 3, His Tag, E. coli inhibited IL-4-induced HT-2 cell proliferation, with the ED₅₀ at 43 pg/mL.

Citations

No references are available