| Background |
Human CD40L (CD154) is one member of TNF family which is expressed on T cells. It binds the CD40, which is on the antigen-presenting cells (ex: B cells, macrophages and monocytes) Human CD40L is a 21.6 kDa cytokine with 103 amino acid residues which is a transmembrane glycoprotein. Human CD40L plays an important role in the adaptive immune system which induces the B cell maturation and activation. |
| Synonyms |
CD40 ligand, soluble CD40 Ligand, TNFSF5, TRAP, CD154, Gp39, T-BAM, sCD40 Ligand |
| Uniprot ID |
P29965 |
| Molecular Weight |
The protein has a calculated MW of 16.99 kDa.
The protein migrates as 16 kDa under reducing condition (SDS-PAGE analysis). |
| Expression System |
Escherichia coli |
| Purity |
>95% as determined by SDS-PAGE. |
| Activity |
Measure by its ability to induce IL-8 secretion in human PBMCs.
The ED₅₀ for this effect is <5 ng/mL. |
| Endotoxin Level |
<0.1 EU per 1 μg of the protein by the LAL method. |
| Protein Sequence |
MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL with polyhistidine tag at the C-terminus |
| Protein Tag |
His Tag (C-term) |
| Form |
The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us. |
| Application |
Cell Culture |
No references are available
