无动物源成分重组细胞因子

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重组小鼠粒细胞-巨噬细胞集落刺激因子 /Recombinant GM-CSF，Mouse，Animal-Free

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产品编号	优惠促销	规格	价格	货期
CM060-5MP	买一赠一	5 ug	￥600.00	现货
CM060-20MP	买一赠一	20 ug	￥1500.00	现货
CM060-100MP	买一赠一	100 ug	￥4500.00	现货
CM060-500MP	买一赠一	500 ug	￥13000.00	请询
CM060-1000MP	买一赠一	1 mg	￥20000.00	请询

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产品简介

背景描述	Granulocyte-macrophage colony-stimulating factor (GM-CSF) was first identified as a Growth Factors due to its ability to induce proliferation and differentiation of bone marrow progenitors into granulocytes and macrophages. GM-CSF is produced by multiple cell types including activated T cells, B cells, macrophages, endothelial cells and fibroblasts upon receiving immune stimuli. GM-CSF stimulates stem cells to produce granulocytes and monocytes functions as a cytokine.
别称	granulocyte-macrophage colony-stimulating factor, colony stimulating factor 2, CSF2, CSF, Csfg, Csfgm, GMCS, GMCSF, Gm-CS, Gm-CSf, MGI-I, MGI-IGM
蛋白编码	P01587
分子量	The protein has a calculated MW of 15.1 kDa. The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>98% as determined by SDS-PAGE.
生物活性	Measure by its ability to induce proliferation in FDC-P1 cells. The ED₅₀ for this effect is <50 pg/mL. The specific activity of recombinant mouse GM-CSF is approximately >2 x 10⁷ IU/mg.
内毒素检测	<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列	APTRSPITVTRPWKHVEAIKEALNLLDDMPVTLNEEVEVVSNEFSFKKLTCVQTRLKIFEQGLRGNFTKLKGALNMTASYYQTYCPPTPETDCETQVTTYADFIDSLKTFLTDIPFECKKPVQK with polyhistidine tag at the N-terminus.
蛋白标签	His Tag (N-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示

SDS- PAGE analysis of recombinant mouse GM-CSF

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