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重组人/小鼠/大鼠激活素A /Recombinant Activin A,Human/Mouse/Rat,Animal-Free
产品编号 优惠促销 Alternate Text 规格 价格 货期
CM118-5HP 买大送小 5 μg ¥600.00 现货
CM118-20HP 买大送小 20 μg ¥1500.00 现货
CM118-100HP 100 μg ¥3980.00 现货
CM118-500HP 500 μg ¥9980.00 请询
CM118-1000HP 1 mg ¥16980.00 请询
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产品简介
背景描述 Activin is the member of the TGF beta superfamily of cytokines. The current understanding of Activin A is classified as a hormone, a Growth Factors, and a cytokine. Depending on the different cell type, that overexpression of Activin A can either inhibit or promote cells division. There are important implications for tumor biology. Activin A also increase joint inflammation in rheumatoid arthritis (RA) and induces pathogenic mechanisms in the respiratory system.
别称 Inhibin beta A chain, Activin beta-A chain, Erythroid differentiation protein
蛋白编码 P08476(Human), Q04998 (Mouse), P18331(Rat)
分子量 The protein has a calculated MW of 13.1 kDa. The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).
表达系统 Escherichia coli
纯度 >95% as determined by SDS-PAGE.
生物活性 Measure by its ability to inhibit the proliferation of mouse MPC-11 cells. The ED₅₀ for this effect is <10 ng/mL. The specific activity of recombinant human Activin A is approximately >1.0 x 10³ IU/mg.
内毒素检测 <0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列 GLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS
蛋白标签 Tag Free
产品形式 The protein was lyophilized from a 0.2 µm filtered solution containing 50 mM Tris, 500 mM NaCl, pH 8.5. If you have any concerns or special requirements, please confirm with us.
产品应用 Cell Culture
产品使用
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 100-200 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
产品储存/运输
储存

Lyophilized protein: Store at -20°C for 1 year.

After reconstitution:

· Store at 2°C to 8°C for up to 1 week.

· For extended storage (beyond 1 week), dilute the reconstituted protein in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/ml. Prepare aliquots (≥20 μl) and store at -20°C or -80°C for 3 to 6 months.

Avoid repeated freeze-thaw cycles.

运输 Blue Ice
实验数据展示
SDS- PAGE analysis of recombinant human/mouse/rat Activin A
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