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重组人/小鼠/大鼠激活素A /Recombinant Activin A,Human/Mouse/Rat,Animal-Free
产品编号 优惠促销 Alternate Text 规格 价格 货期
CM118-5HP 买一赠一 5 ug ¥600.00 现货
CM118-20HP 买一赠一 20 ug ¥1500.00 现货
CM118-100HP 100 ug ¥4500.00 现货
CM118-500HP 500 ug ¥13000.00 请询
CM118-1000HP 1 mg ¥20000.00 请询
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产品简介
背景描述 Activin is the member of the TGF beta superfamily of cytokines. The current understanding of Activin A is classified as a hormone, a Growth Factors, and a cytokine. Depending on the different cell type, that overexpression of Activin A can either inhibit or promote cells division. There are important implications for tumor biology. Activin A also increase joint inflammation in rheumatoid arthritis (RA) and induces pathogenic mechanisms in the respiratory system.
别称 Inhibin beta A chain, Activin beta-A chain, Erythroid differentiation protein
蛋白编码 P08476(Human), Q04998 (Mouse), P18331(Rat)
分子量 The protein has a calculated MW of 13.1 kDa. The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).
表达系统 Escherichia coli
纯度 >95% as determined by SDS-PAGE.
生物活性 Measure by its ability to inhibit the proliferation of mouse MPC-11 cells. The ED₅₀ for this effect is <10 ng/mL. The specific activity of recombinant human Activin A is approximately >1.0 x 10³ IU/mg.
内毒素检测 <0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列 GLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS
蛋白标签 Tag Free
产品形式 The protein was lyophilized from a 0.2 µm filtered solution containing 0.1M Glycine, 150mM NaCl, pH 4.0. If you have any concerns or special requirements, please confirm with us.
产品应用 Cell Culture
产品储存/运输
储存 Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输 Blue Ice
产品使用
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
实验数据展示
SDS- PAGE analysis of recombinant human/mouse/rat Activin A
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