重组人(哺乳动物细胞表达)高迁移率族蛋白B1 /Recombinant HMGB1 C23AC45A,Human,Animal-Free
产品编号 优惠促销 Alternate Text 规格 价格 货期
CM176-5HP 5 μg ¥1000.00 现货
CM176-20HP 20 μg ¥2450.00 现货
CM176-100HP 100 μg ¥4700.00 现货
CM176-500HP 500 μg ¥18500.00 请询
CM176-1000HP 1 mg ¥32000.00 请询
产品简介
背景描述 High Mobility Group protein B1 protein (HMGB1) is the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 25 kDa single chain polypeptide containing three domains: two N-terminal HMG boxes A and B, and a negatively charged 30 a.a. C-terminal region that contains only Asp and Glu. Post-translational modification on HMGB1 have been reported, affects its localization, receptor interactions, and function. HMGB1, with a disulfide bond between C23 and C45 , have been reported that cause cytokine production and the activation of NF-κB. Therefore, we developed the HMGB1 C23A& C45A mutant proteins, eliminant the disulfide bond formation.
别称 high mobility group box 1, HMG-1, HMG1, HMG3, SBP-1
蛋白编码 P09429
分子量 The protein has a calculated MW of 36.36 kDa. The protein migrates as 35-48 kDa under reducing condition (SDS-PAGE analysis).
表达系统 HEK293
纯度 >98% as determined by SDS-PAGE.
生物活性 Measure by its ability to induce TNF alpha in RAW264.7 cells. The ED₅₀ for this effect is <10 μg/mL.
内毒素检测 <0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列 MGKGDPKKPRGKMSSYAFFVQTAREEHKKKHPDASVNFSEFSKKASERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEDEEEDDDDE with polyhistidine-SUMO tag at the N-terminus
蛋白标签 His-SUMO Tag (N-term)
产品形式 The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
产品应用 Cell Culture
产品使用

1. Before opening, centrifuge at 3000 rpm for 5 mins.


2.Initial Reconstitution

· Reconstitute the Lyophilized Protein in sterile H₂O to a concentration of 100-200 μg/mL.

· Then, incubate it at room temperature for at least 20 mins to ensure sufficient dissolution.

   Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

· Store at 2°C to 8°C for up to 1 week.


3.Extended Storage

· After the initial reconstitution, further dilute the reconstituted protein in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/ml.

· Prepare aliquots (≥20 μl).

· Store at -20°C or -80°C for 3 to 6 months.

   Avoid repeated freeze-thaw cycles.

产品储存/运输
储存

Lyophilized Protein: Store at -20°C for 1 year.

Initial Reconstitution: Store at 2°C to 8°C for up to 1 week.

Extended Storage: Store at -20°C or -80°C for 3 to 6 months with a carrier protein or stabilizer.

运输 Blue Ice
实验数据展示
SDS- PAGE analysis of recombinant human HMGB1 C23AC45A
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