重组人4-1BB配体 /Recombinant 4-1BBL,Human,Animal-Free
产品编号 规格 价格 货期
CM085-5HP 5 ug ¥600.00 现货
CM085-20HP 20 ug ¥1500.00 现货
CM085-100HP 100 ug ¥4500.00 现货
CM085-500HP 500 ug ¥13000.00 请询
CM085-1000HP 1 mg ¥20000.00 请询
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产品简介
背景描述 4-1BB ligand (4-1BBL) is a type II transmembrane protein that is part of the tumor necrosis factor (TNF) ligand family. As an inducible co-stimulatory molecule, it presents on several antigen presenting cell (APC) types, including B cells, macrophages and DCs. The interactions between 4-1BB and 4-1BBL trigger pleiotropic effects on the immune response including antigen presenting process, proliferation of CD4 and CD8 positive T-cells, as well as cytokine secretion from T-cells through NFκB, c-Jun, and p38 downstream signal pathways activation. Therefore, 4-1BB and 4-1BBL are recently used for the immunotherapy of cancer.
别称 4-1BB ligand, CD137L, TNLG5A, TNFSF9, Tumor necrosis factor ligand superfamily member 9
蛋白编码 P41273
分子量 The protein has a calculated MW of 20.4 kDa. The protein migrates as 20 kDa under reducing condition (SDS-PAGE analysis).
表达系统 Escherichia coli
纯度 >95% as determined by SDS-PAGE.
生物活性 Measure by its ability to induce IL-8 secretion in human PBMCs. The ED₅₀ for this effect is 1-5 ng/mL.
内毒素检测 <0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列 MREGPELSPDDPAGLLDLRQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVTPEIPAGLPSPRSE with polyhistidine tag at the C-terminus.
蛋白标签 His Tag (C-term)
产品形式 The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
产品应用 Cell Culture
产品储存/运输
储存 Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输 Blue Ice
产品使用
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
实验数据展示
SDS- PAGE analysis of recombinant human 4-1BBL
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