GMP级别细胞因子

重组人肿瘤坏死因子alpha / Recombinant TNF alpha，Human，GMP

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产品编号	规格	价格	货期
CM046-100HPG	100 ug	￥2650.00	请询
CM046-1000HPG	1 mg	￥19500.00	请询

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产品简介

背景描述	Tumor necrosis factor alpha (TNF alpha) stimulates the acute phase of the immune response. In response to a pathogen, TNF alpha is one of the first to be released and can apply its effects in many organs. TNF alpha stimulates the release of corticotropic releasing hormone, suppresses appetite, and induces fever, in the hypothalamus. TNF increase vasodilation and loss of vascular permeability, it helps recruit lymphocyte, neutrophil, and monocyte to the inflammation site by regulating chemokine release.
别称	tumor necrosis factor beta, TNFSF1B, Lymphotoxin-alpha (LT-α),LTA
蛋白编码	P01375
分子量	The protein has a calculated MW of 18.3 kDa. The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>97% as determined by SDS-PAGE analysis.
生物活性	Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED₅₀ for this effect is < 0.1 ng/mL. The specific activity of recombinant human TNF alpha is approximately ≧ 1 x 10⁷ IU/mg, which is calibrated against the human TNF Alpha WHO International Standard (NIBSC code: 12/154).
内毒素检测	<0.05 EU per 1 μg of the protein by the LAL method.
蛋白序列	MVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL with polyhistidine tag at the C-terminus.
蛋白标签	His Tag (C-term)
产品形式	Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution at RT for at least 20 min to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示

SDS- PAGE analysis of GMP human TNF alpha

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