GMP级别细胞因子

重组人白细胞介素7 / Recombinant IL-7，Human，GMP

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产品编号	规格	价格	货期
CM009-100HPG	100 ug	￥11500.00	请询
CM009-1000HPG	1 mg	￥52000.00	请询

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产品简介

背景描述	Interleukin-7 (IL-7) is a multipotent cytokine belonged to one of the members of IL-2 superfamily. IL-7 has diverse effects on the hematopoietic and immune regulations. IL-7 is a trophic factor that is necessary for both B cell and T cell proliferation and development. In addition, it presents potential antitumor effects in tumors such as glioma, melanoma, lymphoma, leukemia, prostate cancer, and glioblastoma.
别称	interleukin 7, Lymphopoietin 1(LP-1), pre-B-cell factor, IL7
蛋白编码	P13232
分子量	The protein has a calculated MW of 18.3 kDa. The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>95% as determined by SDS-PAGE analysis.
生物活性	Measured by its ability to induce PHA-activated human PBMCs proliferation. The ED₅₀ for this effect is <0.8 ng/mL. The specific activity of recombinant human IL-7 is > 1 x 10⁸ units/mg.
内毒素检测	<0.05 EU per 1 μg of the protein by the LAL method.
蛋白序列	MDCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH with polyhistidine tag at the C-terminus.
蛋白标签	His Tag (C-term)
产品形式	Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution at RT for at least 20 min to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示


SDS- PAGE analysis of GMP human IL-7	GMP Human IL-7 Protein, His Tag, E. coli stimulates the proliferation of PHA-activated human peripheral blood lymphocytes (PBMC), with the ED₅₀ < 0.8 ng/mL.

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