产品形式 | Liquid |
产品应用 | ELISA,WB |
产品描述 | AccuBlot™ Anti-Rabbit IgG (H+L) Antibody (HRP) is conjugated with high-activity HRP. In coimmunoprecipitation (co-IP) assay, denatured IgG from IP samples may show interference and result in immunoblotting at ~55 kDa (heavy chain) and ~25 kDa (light chain) by using traditional secondary antibodies. The products of AccuBlot™ series are suitable for native IgG detection. |
反应性 | Rabbit IgG |
克隆性 | Monoclonal |
免疫原 | Purified rabbit IgG |
浓度 | Lot-dependent, please refer to product label. |
标记 | HRP |
产品组成 | Phosphate Buffered Saline containing 1% BSA, 0.03% ProClin 300 and 50% glycerol, pH 7.4. |
储存 | This product is stable after storage at: 2-8°C for 2 weeks under sterile conditions from date of receipt. -20°C or -80°C for 12 months under sterile conditions from date of receipt. Avoid repeated freeze/thaw cycles and protect from light. Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C. |
运输 | The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage. |
Recommended dilution factor:
ELISA: 1:2500-10000
WB:1:1000-3000
Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.