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重组人R-脊椎蛋白1 /Recombinant R-spondin 1,Human,Animal-Free
产品编号 优惠促销 Alternate Text 规格 价格 货期
CM181-5HP 买大送小 5 μg ¥600.00 现货
CM181-20HP 买大送小 20 μg ¥1500.00 现货
CM181-100HP 买大送小 100 μg ¥3980.00 现货
CM181-500HP 买大送小 500 μg ¥9980.00 请询
CM181-1000HP 买大送小 1 mg ¥16980.00 请询
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产品简介
背景描述 R-spondin 1 (RSPO1, Roof plate-specific Spondin 1) is a cysteine-rich secreted glycoprotein that belongs to the R-Spondin family. Structurally, R-spondin 1 harbors an N-terminal signal peptide, two cysteine-rich furin-like (FU1-FU2) domains, a thrombospondin (TSP) domain, and a basic amino acid-rich (BR) C-terminal domain. R-spondin 1 acts as a ligand for the leucine-rich repeat-containing G-protein coupled receptor (LGR) family (LGR4/5/6), subsequent clearing negative regulators ZNRF3/RNF43 from the membrane, leading to the availability of Wnt receptor and activating Wnt/β-catenin signaling. The signal cascade is essential for regulating stem cell proliferation, embryonic development, and tissue homeostasis.
别称 RSPO1, Roof plate-specific spondin-1
蛋白编码 Q2MKA7
分子量 The protein has a calculated MW of 46.8 kDa. The protein migrates as 62-68 kDa under reducing condition (SDS-PAGE analysis).
表达系统 HEK293
纯度 >95% as determined by SDS-PAGE.
生物活性 R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED₅₀ for this effect is < 3 μg/mL.
内毒素检测 <1 EU per 1 μg of the protein by the LAL method.
蛋白序列 A DNA sequence encoding Human R-Spondin 1 Protein (#Q2MKA7) (Ser21 - Ala263) was expressed with polyhistidine-SUMO tag at the N-terminus.
蛋白标签 His-SUMO Tag (N-term)
产品形式 Liquid (1X PBS, pH 7.4) If you have any concerns or special requirements, please confirm with us.
产品应用 Cell Culture
浓度 Please refer to the product label.
产品使用
Centrifuge at 3000 rpm for 5 mins before opening. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
产品储存/运输
储存

Stock solution: Store at 2°C to 8°C for up to 1 week, -20°C or -80°C for 12 months.

After dilution: Dilute the stock solution in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/ml. Prepare aliquots (≥20 μl) and store at -20°C or -80°C for 3 to 6 months.

Avoid repeated freeze-thaw cycles.

运输 Blue Ice
实验数据展示
SDS- PAGE analysis of recombinant human R-spondin 1
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