| 背景描述 | N-Acetylcysteine (NAC) is a multifunctional thiol compound and precursor of glutathione (GSH), the primary intracellular antioxidant. In cell and organoid culture, NAC mitigates oxidative stress induced by reactive oxygen species (ROS), which often arises during dissociation, thawing, or long-term culture. It protects cell membranes, preserves DNA integrity, and reduces apoptosis, thereby improving cell survival and culture stability. NAC is also known to modulate inflammatory pathways and enhance the functional maturation of organoids (e.g., liver, lung, and kidney organoids), making it a versatile supplement in regenerative medicine research. |
| 纯度 | 98% |
| 生物活性 | Acetylcysteine is a thiol-containing antioxidant that has been shown to inhibit the binding activity of NF-κB in response to multiple agonists in various cell types.Preincubation of human umbilical vein endothe lial cells with acetylcysteine (0 ‒ 40mM) for 1h dose-dependently inhibited IL-1-induced monocytic cell adhesion. The IC50 value of acetylcysteine was 20mM, and the 95% inhibition was observed at 40mM. Acetylcysteine treatment also resulted in a reduction in surface expression of E-selectin and VCAM-1 of 56% and 70%, respectively, in endothelial cells. |
| 产品形式 | White Power |
| CAS号 | 616-91-1 |
| 分子式 | C5H9NO3S |
| 分子量 | 163.19 |
| SMILES | S([H])C([H])([H])[C@@]([H])(C(=O)O[H])N([H])C(C([H])([H])[H])=O |
| MDL | MFCD00004880 |
| 溶解度 | DMSO：105 mg/mL(643.4 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO。 H2O：100 mg/mL(612.76 mM)，配合低频超声助溶。 Acetylcysteine用于细胞实验纯水配制母液后用培养基稀释到工作液浓度时培养基的pH可能偏酸，若培养基的pH偏酸需要用氢氧化钠将pH调至培养条件。 |
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| 储存 | Pure form: Keep in dark place, sealed in dry, room temperature. In solvent: -20°C for 3-6 months, -80°C for 12 months. |
| 运输 | Blue Ice |
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