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Protein expression and purification (E. coli  system)

♦ Key service features:

• Using Chamot’s exclusive protein expression technique, we are able to raise successful protein expression rate to above 98%.
• Flexibility for client requirement, and the small-scale production could be executed before large scale production.
• Experienced in resolving of common problem, such as insoluble protein or low yield of production.
• We also provide other additional services, such as endotoxin detection, bio-activity analytic platform, tag protein removal, protein sub package and lyophilization.
• One-step service from codon optimization to protein purification.

♦ Service workflow:

• The sequence and protein information of interested targets should be provided.
• Gene synthesis and plasmid production.
• Plasmid transfection into expression E. coli strain.
• Protein expression optimization.
• Protein purification
• QC analysis

♦ Sample requirements:

• Target protein sequence
• Requirement of target protein

♦ Deliverables:

• Purified protein and final report (including SDS-PAGE, Western blot).

Protein expression and purification (Mammalian cell system)

♦ Key service features:

• Using Chamot’s exclusive protein expression technique, the expression success rate of the target recombinant proteins can increase to above 90%.
• Flexibility for clients’ requirements, and the small scale production could be executed before large scale production.
• We also provide other additional services such as endotoxin detection, bio-activity analysis platform, tag removal and lyophilization.
• One-stop service from codon analysis to  protein purification.

♦ Service workflow:

• Clients provide sequence and protein information of interested target.
• Gene synthesis and plasmid production.
• Plasmid transfection into mammalian cell.
• Protein expression optimization.
• Protein purification.
• Provide purified target protein with analysis report.

♦ Sample requirements:

• Target protein sequence
• Requirement of target protein

♦ Deliverables:

• Purified protein and final report (including SDS-PAGE, Western blot).