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Protein expression and purification (E. coli system)
♦ Key service features:
- Using Chamot’s exclusive protein expression technique, we are able to raise successful protein expression rate to above 98%.
- Flexibility for client requirement, and the small-scale production could be executed before large scale production.
- Experienced in resolving of common problem, such as insoluble protein or low yield of production.
- We also provide other additional services, such as endotoxin detection, bio-activity analytic platform, tag protein removal, protein sub package and lyophilization.
- One-step service from codon optimization to protein purification.
♦ Service workflow:
- The sequence and protein information of interested targets should be provided.
- Gene synthesis and plasmid production.
- Plasmid transfection into expression E. coli strain.
- Protein expression optimization.
- Protein purification
- QC analysis
♦ Sample requirements:
- Target protein sequence
- Requirement of target protein
♦ Deliverables:
- Purified protein and final report (including SDS-PAGE, Western blot).
Protein expression and purification (Mammalian cell system)
♦ Key service features:
- Using Chamot’s exclusive protein expression technique, the expression success rate of the target recombinant proteins can increase to above 90%.
- Flexibility for clients’ requirements, and the small scale production could be executed before large scale production.
- We also provide other additional services such as endotoxin detection, bio-activity analysis platform, tag removal and lyophilization.
- One-stop service from codon analysis to protein purification.
♦ Service workflow:
- Clients provide sequence and protein information of interested target.
- Gene synthesis and plasmid production.
- Plasmid transfection into mammalian cell.
- Protein expression optimization.
- Protein purification.
- Provide purified target protein with analysis report.
♦ Sample requirements:
- Target protein sequence
- Requirement of target protein
♦ Deliverables:
- Purified protein and final report (including SDS-PAGE, Western blot).