Hybridoma sequencing service
♦ Key service features:
- Chamot Biotechnologyis dedicated to antibody development and has established a high-accuracy sequencing platform to provide extraordinary sequencing service.
- We promise that all information will be kept confidential within Chamot Biotechnology.
- Furthermore, we provide a customized service for recombinant antibody production according to clients’requirements.
♦ Service workflow:
- Clients provide the target hybridoma cell line.
- RNA extraction from hybridoma cells
- Reverse transcription PCR
- Vector construction
- Sequence analysis
♦ Sample requirements:
- The number of hybridoma cells: > 5 x 10^6 per tube (Please ensure that the cells are healthy.)
- Clients are encouraged to provide the information of hybridoma cells as detailed as possible they can, including cell species, cell source, fusion partner, antibody isotype, culture condition, and duration of cryopreservation.
- Sequence report of the heavy and light chains in the variable region, including the CDS sequence.