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Recombinant antibody production service(重组抗体定制)
♦ Key service features:
- Professional antibody sequence evaluation and optimization.
- Various constant region backbone choices.
Human: IgG1
Mouse: IgG2b
- Small scale expression test depends on your requirements.
- Flexible choice of antibody expression level of cell culture volume. (50 mL / 100 mL / 400 mL)
♦ Service workflow:
- Antibody sequence evaluation
- Choose the constant region backbone
- Gene synthesis and plasmid construction (VH-CH/VL-CL)
- Antibody expression and purification
♦ Sample requirements:
- Provide the sequence of antibody variable region
♦ Deliverables:
- Expression plasmid (Heavy chain and Light chain)
- Purified antibodies
Antibody humanization service(人源化抗体定制)
♦ Key service features:
- Our dedicated team will provide the most professional and attentive sequence prediction of the humanized antibody.
- We offer Biacore T200 service for clients that help you understand the binding affinity of your humanized antibody. (clients need to provide antigen)
♦ Service workflow:
- Clients need to provide the sequence of variable region.
- The gene structure of antibody VH and VL sequence will be analyzed by our experts.
- Chimeric antibody production, which contain mouse V region and human C region.
- Correct the sequence of humanized antibody with matching to human germline template.
- We will design and produce multiple candidates of humanized antibodies.
- We will pick up the best clone of these candidates depend on the test results (ELISA).
♦ Sample requirements:
- Sequence of variable region.
- Antigen for binding affinity analysis.
♦ Deliverables:
- Final report (including sequence analysis, results of binding affinity analysis result).
- Humanized antibody