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Mouse monoclonal antibody development service(鼠单抗定制)
♦ Key service features:
- Our experts will offer professional analysis and suggestions for antigen design.
- Our capable project manager and scientists will support and discuss with you throughout the process.
- The antibody screening service can be customized according to the client’s requirements.
♦ Service workflow:
- Phase I :Antigen design and evaluation.
- Phase II :Four BALB/c mice immunization and then titration of antibodies in sera by ELISA.
- Phase IIIa:Hybridoma preparation and screening.
- Phase IIIb:Hybridoma stabilization and expansion.
- Phase IVa:Ascites induction and monoclonal antibody production.
- Phase IVb:Antibody purified by protein A/G.
♦ Sample requirements:
- The sequence and information of the target peptide or protein antigen are required. (Phase I)
- We will offer clients sera of antigen-immunized mice to determine the mice candidates for hybridoma preparation. (Phase II)
- Client can select 1-3 candidates for the following clones stabilization and expansion. (Phase III)
♦ Deliverables:
- 2 vials of cryogenic tubes/hybridoma clones.
- 5 mL supernatant collected from hybridoma culture media/ hybridoma clones.
- QC report of indirect ELISA .
Rabbit polyclonal antibody development service(兔多抗定制)
♦ Key service features:
- Our experts will offer professional analysis and suggestion for antigen design.
- Flexibility to increase the number of immunized animals.
- We provide professional affinity purification technique to increase the purity of antibodies.
♦ Service workflow:
- Antigen design and evaluation
- Carrier protein-conjugated peptide synthesis or customized protein production (depending on clients’ requirements)
- Two New Zealand Rabbit immunization with your target antigen
- Collecting the serum from each of antigen-immunized rabbit
- Improving the specificity and affinity of antibodies by affinity purification
- Binding ability test of antibody to immunogen by indirect ELISA test and QC report
♦ Sample requirements:
- Clients should offer the sequence for their target peptide or protein immunogen.
- If clients prefer to use their own protein as immunogens, the following condition should have complied.
Protein: concentration ≧ 1 mg/mL, Amount ≧ 5 mg, Purity > 90%.
* If the clients want to do an affinity purification, clients should provide at least additional 5 mg antigen. (8-10 mg total)
♦ Deliverables:
- 1 mg peptide or X mg protein immunogen (depend on client requirement)
- 2-10 mg purified rabbit polyclonal antibody
- Report (with indirect ELISA analysis)