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Mouse monoclonal antibody development service（鼠单抗定制）

♦ Key service features:

• Our experts will offer professional analysis and suggestions for antigen design.
• Our capable project manager and scientists will support and discuss with you throughout the process.
• The antibody screening service can be customized according to the client’s requirements.

♦ Service workflow:

• Phase I ：Antigen design and evaluation.
• Phase II ：Four BALB/c mice immunization and then titration of antibodies in sera by ELISA.
• Phase IIIa：Hybridoma preparation and screening.
• Phase IIIb：Hybridoma stabilization and expansion.
• Phase IVa：Ascites induction and monoclonal antibody production.
• Phase IVb：Antibody purified by protein A/G.

♦ Sample requirements:

• The sequence and information of the target peptide or protein antigen are required. (Phase I)
• We will offer clients sera of antigen-immunized mice to determine the mice candidates for hybridoma preparation. (Phase II)
• Client can select 1-3 candidates for the following clones stabilization and expansion. (Phase III)

♦ Deliverables:

• 2 vials of cryogenic tubes/hybridoma clones.
• 5 mL supernatant collected from hybridoma culture media/ hybridoma clones.
• QC report of indirect ELISA  .

Rabbit polyclonal antibody development service（兔多抗定制）

♦ Key service features:

• Our experts will offer professional analysis and suggestion for antigen design.
• Flexibility to increase the number of immunized animals.
• We provide professional affinity purification technique to increase the purity of antibodies.

♦ Service workflow:

• Antigen design and evaluation
• Carrier protein-conjugated peptide synthesis or customized protein production (depending on clients’ requirements)
• Two New Zealand Rabbit immunization with your target antigen
• Collecting the serum from each of antigen-immunized rabbit
• Improving the specificity and affinity of antibodies by affinity purification
• Binding ability test of antibody to immunogen by indirect ELISA test and QC report

♦ Sample requirements:

• Clients should offer the sequence for their target peptide or protein immunogen.
• If clients prefer to use their own protein as immunogens, the following condition should have complied.

  Protein: concentration ≧ 1 mg/mL, Amount ≧ 5 mg, Purity > 90%.

* If the clients want to do an affinity purification, clients should provide at least additional 5 mg antigen. (8-10 mg total)

♦ Deliverables:

• 1 mg peptide or X mg protein immunogen (depend on client requirement)
• 2-10 mg purified rabbit polyclonal antibody
• Report (with indirect ELISA analysis)